Pdf qtl mapping in maize

Qtl mapping and epistasis analysis of brace root traits in maize. Map overlapping of qtl for resistance to fusarium ear rot. Qtl mapping and prediction of haploid male fertility. Aug 01, 2020 for root angle, qtl mapping has been conducted in maize, rice, and sorghum. Kernel size is an important agronomic trait for grain yield in maize. Qtl b73 x b52 species were in commonacross three mapping popula 3. Mapping quantitative trait loci for yieldrelated traits and predicting. Pdf qtl mapping of fusarium moniliforme ear rot resistance. Otherwise, the linked nontarget qtl will disturb the fine mapping of the target qtl.

Seven out of 10 qtl were pleiotropic to both diatraea spp. Improving spontaneous haploid genome doubling shgd is an alternative to overcome this limitation. Haploid genome doubling is a critical step in dh breeding. Qtl mapping and candidate genes for resistance to fusarium. Traditional fine mapping of the causal gene for complex trait, based on the transition from the quantitative trait locus qtl to a single qualitative gene through backcrossing or the construction of heterogeneous inbred family hif, has achieved great success in model crop rice. Qtl mapping of quantitative traits in maize request pdf. Plants homozygous for the teosinte allele at tb1 showed greater phenotypic plasticity across environments than plants homozygous for the maize allele. Qtl mapping in three tropical maize populations reveals a. Qtl mapping and candidate genes for resistance to fusarium ear rot and fumonisin contamination in maize valentina maschietto1, cinzia colombi2, raul pirona2,3, giorgio pea2, francesco strozzi2, adriano marocco1, laura rossini2,4 and alessandra lanubile1 abstract.

Genomewide association study and qtl mapping reveal genomic. Nov 01, 2018 otherwise, the linked nontarget qtl will disturb the fine mapping of the target qtl. Apr 01, 2017 multiple interval mapping mim, an extension of interval mapping to multiple qtl, tends to be more powerful and precise than cim in identifying qtl and allows the simultaneous estimation of multiple qtl with epistasis. Mapping qtl for disease resistance and associated traits is important to develop maize zea mays l. A bc 2 s 3 maize teosinte ril population for qtl mapping laura m. Oct 26, 2011 ten qtl in the rils and 15 qtl in the if2 population were detected.

Qtl mapping for maize starch content and candidate gene. In this study, the elite maize inbred line zheng6831 was used as a recurrent parent and the four maize inbred lines zph88, zph5, dong 237 and chang 72 were used as donor parents. The location of the qtl was carried out through the analysis by the composite interval mapping cim, using the qtl cartographer software basten et al. It is, therefore, vital for maize breeding programs to improve tolerance to low temperatures at seed germination stage. Qtl mapping provides usefull information for breeding programs since it allows the estimation of genomic locations and genetic effects of chromossomal.

Qtl analysis of pi leaf performance index detected three leaf function qtls on chromosomes 4, 8, and 9 were related to gls resistance and explained 4. Maize genetics cooperation newsletter shannon 1 1 please note. Qtl mapping for leaf morphology traits in a large maize. Jumbo 2, pangirayi tongoona 3, eric danquah 3, beatrice e. Pdf optical topometry and machine learning to rapidly. However, little is known about maize qtl involved in lowtemperature germination ability. Qtl mapping analysis of maize plant type based on snp molecular marker cell mol biol noisylegrand.

Identification of qtls for grain yield and other traits in tropical maize. C orn and its wild progenitor, teosinte, differ dramatically in their overall plant architecture and the morphology of their female in. Qtl mapping of lowtemperature germination ability in the. Multienvironment qtl mapping of crown root traits in a.

Return to article details qtl mapping linked to downy mildew resistance genes in maize zea mays download download pdf. Qtl mapping for yield components in a tropical maize population. Map overlapping of qtl for resistance to fusarium ear rot and. Mapping quantitative trait loci for resistance to fall. Doebley1, 1 laboratory of genetics, university of wisconsinmadison, wi 53706, usa. Genetic analysis of agronomic traits associated with plant. Germplasm resources for mapping 10 quantitative traits in maize. Using a bc1f1 backcross mapping population derived from a cross between 1145 donor parent, completely resistant and y331 recurrent parent, highl. Genome doubling of haploids is one of the major constraints of largescale doubled haploid dh technology. Fusarium graminearum schwabe, the conidial form of gibberella zeae, is the causal fungal pathogen responsible for gibberella stalk rot of maize. Quantitative trait locus qtl mapping studies in maize indicated that fusarium resistance and fumonisin contamination are quantitative traits determined by small effect polygenes 1518. Thus, qtl mapping in tropical maize germplasm could identify qtl not present in temperate germplasm, and because of the high genotype by environment interaction in tropical areas, this type of research should focus not only on mapping qtl but, also, on qtl by environment qtl e interaction.

The introgression this qtl can overcome the need of colchicine in dh lines development. Qtl mapping and gene expression analyses reveal that pstol candidate genes are associated with root morphology and phosphorus acquisition in maize. Epistatic and environmental interactions for quantitative. Apr 01, 2020 in vivo doubled haploid dh technology is widely used in commercial maize zea mays l.

The four f1 hybrids were produced by crossing between them and were continued backcrossing to the recurrent paren. Previous studies of qtl mapping of maize agronomic traits focused on f 2 ribaut et al. The highly embryogenic and regenerable maize germplasm, hiiia. Qtl qtlregions with effects on all three maize stem borer 1. Awata 1,2,3, yoseph beyene 2, manje gowda 2, suresh l. In maize, some traits at the seedling stage were conducted for mapping of qtl associated with chilling tolerance, such as chlorophyll fluorescence parameters, leaf greenness, leaf area, shoot dry weight, and shoot nitrogen content. Qtl mapping strategies have also generated finescale genetic maps to undertake markerassisted selection in breeding programs and positional cloning aranza et al. However, haploid genome doubling is a bottleneck for the commercialization of dh technology. Qtl mapping of resistance to fusarium ear rot using a ril.

Traditional fine mapping of the causal gene for complex trait, based on the transition from the quantitative trait locus qtl to a single qualitative. Pdf mapping qtl for grain yield and plant traits in a. Jun 28, 2019 here, using a large population of 866 maize teosinte bc 2 s 3 recombinant inbred lines genotyped with 19,838 snp markers, we performed highresolution quantitative trait locus qtl mapping for three leaf morphological traits, including leaf length, leaf width, and sheath length. Perezbrito and colleagues 15 identified 16 qtls for fer resistance in two f 2. Mapping prolificacy qtl in maize and teosinte journal of. Qtl mapping in a maize f 2 population using genotypingbysequencing and a modified fine mapping strategy plant sci. Moreover, the ratio of superior allele at each significant snp ranged from 0 to 83. Multienvironment qtl mapping of crown root traits in a maize.

The purpose of this chapter is to provide a brief overview of qtl mapping methods, to provide background about commonly used germplasm resources, and to discuss the strengths and weakness of each. The objectives of this work were to identify genomic regions associated with. Southwestern corn borer and sugarcane borer diatraea saccharalis f. Genetic mapping of quantitative trait loci in crops. Qtl mapping to anthracnose leaf blight resistance in. A bc2s3 maizeteosinte ril population for qtl mapping. View of qtl mapping linked to downy mildew resistance genes in. Three genomic regions, on chromosomes 5 and 9, were consistent with a second mapping population derived from cross ki3 susceptible. Magill sorghum downy mildew sdm of maize is caused by the oomycete peronosclerospora sorghi weston and uppal c. In this study, an f 2 population derived from a maize landrace and an elite inbred line was firstly genotyped by genotyping by sequencing gbs. Qtl mapping studies in maize teosinte f 2 populations have been utilized to determine the number, effect, and. Authors weihong zhu 1, yikun zhao 1, jingbao liu 2, lu huang 2, xiaomin lu 2, dingming kang 1 affiliations 1 moe key laboratory of crop heterosis and. This kind of population has a deficiency, namely, temporality, similar to a backcross. Pdf qtl mapping of plant height and ear position in maize.

The comparative analysis based on maize integrated qtl map and. Qtl mapping approaches are one of the popular genomic tools to dissect the genetic architecture of complex traits 4. Qtl mapping in a maize f 2 population using genotypingby. G c a t genes t a c g g c a t article genetic analysis of qtl for resistance to maize lethal necrosis in multiple mapping populations luka a. Pdf genetic analysis and major qtl detection for maize kernel. Maize landraces possess tremendous diversity, most of maize landraces are locally adapted and have distinct alleles with low frequency arose in the specific regions. Genetic linkage maps and quantitative trait loci qtl. Linkage mapping of domestication loci in a large maize. Qtl mapping of resistance to fusarium ear rot using a ril population in maize junqiang ding.

Analyses of the qtl mapping were carried out using the means of the disease severity grades of the three evaluations and of the audpc of the 121 f 2. Qtl mapping identified 14 qtl with phenotypic variation explained pve ranging from 3. Basic idea cross two distinct lines fat vs skinny mice low vs. Cml9 resistant, for which seven qtl for 1swcb ldr were found. Genetic analysis of qtl for resistance to maize lethal. In two maize teosinte f 2 populations, 10 rootangle qtl were identified, and the qtl on chromosome 7 was found consistently in all populations 21. Qtl analysis further revealed 29 qtls of 12 traits, most of which individually accounted for more than 10% of phenotypic variations. Qtl mapping in maize associated with plant architecture a segregating population must be used for qtl mapping.

Dec 01, 2016 other qtl mapping studies have also revealed many qtl for f. Qtl analysis was performed on four sweet corn germination traits that are related to seed vigor under artificial aging conditions. Qtl mapping in three tropical maize populations reveals a set. The presence of qtl conferring resistance to several viral diseases in maize has been investigated in numerous linkage and linkage disequilibrium mapping studies 48. Using the gwas method, seven snps were identified for fer resistance based on a diverse inbred line population comprised of 1687 maize inbred lines zila et al.

J wang, h li, l zhang, l mengusers manual of qtl icimapping. Mapping of qtl and identification of candidate genes. Abstract a maize genetic linkage map derived from 115 simple sequence repeat ssr markers was constructed from an f2 population. A biparental mapping population of 298 f5 recombinant inbreds ris, obtained from the cross between lp4637 moderately resistant and l4674 susceptible, was genotyped with 250 single nucleotide polymorphism snp markers. Pdf qtl mapping and prediction of haploid male fertility. A large number of software packages implementing the above methods are available for biparental mapping, including qtl. We carried out qtl mapping using snp markers in three tropical populations, involving elite lines across a wide range of environments under contrasting water regimes. Marchelodragga 4, michael olsen 2, veronica ogugo 2, stephen mugo 2 and. Aluminum toxicity is one of the major constraints for. Maize lethal necrosis mln occurs when maize chlorotic mottle. A set of 89 nearisogenic lines nils of maize was created using markerassisted selection. However, now instead of following the gametes from a parent, one looks at a population sample segregating variation exploits linkage disequilibrium between very tightlylinked makers typically 110 kb in humans, maize results in much finer mapping that a qtl linkage analysis, kb versus mb scale. However, this fine mapping strategy did not work well in maize. Construction of highdensity genetic map and identification of qtls.

Qtl mapping for resistance of maize to grey leaf spot he. At the germination stage, germination rate and primary root length are the most important two traits in response. The authors of this mnl submission have granted consent for anyone to cite this work. Genetics of proteome variation for qtl characterization.

Qtl mapping of fusarium moniliforme ear rot resistance in highland maize, mexico daisy perezbrito 1, daniel jeffers 2, diego gonzalezdeleon 2, mireille khairallah 2, moises cortes. Low temperature is the primary factor to affect maize sowing in early spring. Pdf qtl mapping in a maize f2 population using genotypingby. In the public sector, the most highly utilized line for genetic engineering is hiii pronounced high 2, with other lines such as b104, a188, and h99 also used to a lesser degree. A previous genetic map containing 117 microsatellite loci and 400 f2 plants was used for quantitative trait loci qtl mapping in tropical maize. Qtl mapping analysis of maize plant type based on snp. Genetic analysis and qtl mapping of maize yield and associate. Qtl mapping and prediction of haploid male fertility traits. Authors jian wang 1, xuan zhang 1, zhongwei lin 2 affiliations 1 national maize. Gwas studies suggested that gwas has no power to detect minor or rare allele with low frequency. We demonstrate that the three leaf traits were associated with.

The purpose of this study is to map qtls and predict candidate genes for. In the two populations, we identified two coincident major qtl for tbrtn and a single identical major qtl for ebrtn. Qtl mapping of resistance to sorghum downy mildew in maize. Mar 31, 2016 in maize, some traits at the seedling stage were conducted for mapping of qtl associated with chilling tolerance, such as chlorophyll fluorescence parameters, leaf greenness, leaf area, shoot dry weight, and shoot nitrogen content. Qtl mapping and epistasis analysis of brace root traits in. Mapping of qtl for grain yield components based on a dh.

Genomewide association study and qtl mapping reveal. A major qtl for resistance to gibberella stalk rot in maize. Composite interval mapping sh owing the qtls associated with al tolerance in maize at the chromosomes 2 qtl 1, 6 qtl 2, qtl 3 a n d8 qtl 4, qtl 5. Qtls previously detected in other mapping population structures, eshed and zamir 1995 proposed the use of nils to simultaneously map, verify, and incorporate qtls into adapted, elite genetic backgrounds. Germplasm resources for mapping 10 quantitative traits in. Qtl mapping of spontaneous haploid genome doubling using. Qtl mapping approaches are one of the popular genomic tools to. Kernel weight and size are important components of grain yield in cereals. Apr, 2020 a major qtl for shgd was identified on chromosome 5 with stable expression across environments. Analysis of the genetic architecture of maize ear and. Qtl mapping to anthracnose leaf blight resistance in tropical.