Mar 01, 2009 the spermatozoa characteristics of wistar rats in groups a control, b treated with chloramphenicol for 20 days and c treated with chloramphenicol for 25 days are as presented in table i. Gamerman lc, rosenkranz a, schor n, ghiraldini ma, jurkiewicz a, ajzen h, ramos ol. Wibr, hematology values, clinical chemistry values created date. The 9l cell line is a gliosarcoma cell line chemically induced in cd fischer rats 14. This study aimed to investigate the potential sex differences and effect of food intake on the intestinal luminal fluid and the efflux membrane transporter p. Bmc oral health page 3 of 15 considerations used here granted by animal care and use committee under. Summary the spontaneously hypertensive rat shr initially bred in kyoto is the most widely studied animal model of essential hypertension.
Effect of vigrx on testosterone level in male albino wistar rats 30 9. The morphological characteristics and haematological studies were studied in fifteen male albino rats wistar strain aged between 12 and 14 weeks and with a body weight of 150 180g. Characteristics of open field behavior of wistar and. The training was jumping exercise in water environment for 20 days prior to injury, and treatment after the injury. After 14 days oral administration of the saponin extract, the rats. Wistar outbred rats show close genetic similarity with. Limited information between the sexes and the effect of food consumption on the gastrointestinal gi physiology, however, is acknowledged or understood. This study demonstrates the importance of characterizing outbred rats and the need to pay ample attention to the genetic characteristics the slc. In this study a total number of 5 guinea pigs and 5 wistar rats all adult males were used. Mean sem activity scores in wistar, wky, and wkha male rats treated with either saline panel a or 1, 2, or 5 mgkg methylphenidate panels in b prior to exposure to a novel open field. Neurochemical characteristics of the effects of delta sleep. Electrocardiogram delineation in a wistar rat experimental model.
The journal of pharmacology and experimental therapeutics, 322 3, 12081220, 2007. Quantitative cytochemical assay showed that single injection of delta sleepinducing peptide increased monoamine oxidase activity substrates. Wistar rat model information sheet charles river laboratories. Comparative evaluation of the sperm characteristics and morphology of adult wistar rats fed either low or normal proteinenergy diets and orally dosed with aqueous cuscuta australis extracts 1omirinde, j. Forty male rats were randomly distributed into eight experimental groups of ve. As controls for the shr, most workers have used normotensive descendants of wistar rats from the colony in kyoto from which the shr strain was derived wistar kyoto rats, wky. The spontaneously hypertensive rat and the lewis rat are other wellknown stocks developed from wistar rats. Wistar rats given methoxychlor 100 or 200mgkgday for 760 days males or 14. The wistar rat is an outbred stock, used in all fields of medical and biological research. Baseline hematology and clinical chemistry values for charles river wistar rats crl. Morphological characteristics and haematological studies in. The study was conducted to determine the effect of subacute chlorpyrifos exposure on some hormonal and semen characteristics and, the ameliorative effects of melatonin. First, two adult rat groups aged 90 d were studied.
Pdf metabolic syndrome signs in wistar rats submitted to. Therefore, the effects of the maternal hvdiet on tissue mechanisms regulating insulin resistance in offspring part 1 and on characteristics of in the dams mets and their second litter l2 offspring part 2 were examined. Survival at 104 study weeks of both male and female wistar hannover rats. The spraguedawley, fischer 344, wistar, and longevans strains of rats are those most commonly used in research on aging. Comparative morphologic and histological studies on guinea. Laboratory rats introduction origin and development. Longevans rat the longevans rat is an outbred rat developed by drs. Wistar rats 220250 g body weight were purchased from the laboratory animal center of heilongjiang university of chinese medicine harbin, china and divided into two groups group a, drug group for dosed rat plasma, n 7, and group b, control group for blank rat plasma, n 7 and kept in a breeding room. Aug 29, 2019 wistar rats have different characteristics, such as genetic variations and distinct physiological functions, attributable to their specific breed, and background data regarding each wistar strain are important for obtaining accurate results in toxicological and pharmaceutical studies. Spermatozoa morphology and characteristics of male wistar. However, toxicity of nanoparticles is a subject of continued controversy, thus necessitating further studies in this direction. Twelve male wistar rats were grouped into 2 groups of six each control and tartrazinetreated groups. Guinea pigs and wistar rats are currently in use in laboratory research as models for various human medical conditions, for virology and toxicological studies just to mention a few.
It is an albino strain, easytohandle, it is however slower learner than long evans rat. Longevans rats are thought to come from crosses between wistar females and a wild norway rat pass and freeth 1993. Long and evans in 1915 by crossing several wistar females with a wild gray male. Wistar rats are more active than others like sprague dawley rats. Characteristics of spontaneously hypertensive, wistar kyoto. Baseline hematology and clinical chemistry values for charles. The wistar rat, wistar hannover wistar han rat, and wistar unilever wu rat are outbred rat strains, while the wistar kyoto and wistar furth rats are. Hematological characteristics of the bb wistar rat. Spermatozoa morphology and characteristics of male wistar rats administered with ethanolic extract of lagenaria breviflora roberts. Almost all laboratory and domestic pet rats belong to a single species, the norway rat rattus norvegicus. The aim is to study the effect of tartrazine e102 synthetic food colour on the antioxidant status of testis of wistar rats. Hematological characteristics of the bb wistar rat hematological characteristics of the bb wistar rat wright, james r yates, allan j shah, niranjan t neff, john c covey, david w thibert, pierre 19830101 00. The study was conducted with the consent of the institutional ethics committee. Characteristics of open field behavior of wistar and spraguedawley rats.
Quantitative cytochemical analysis of the activities of glucose6phosphate dehydrogenase, aminopeptidase, and glutamate dehydrogenase in the brain of wistar rats with different learning abilities revealed a difference in the level of hippocampal aminopeptidase activity. The data were compared to the wistar rats as agerelated controls. Effect of vigrx on ejaculation frequency in male albino wistar rats 28 7. Intracranial rat glioma models are a useful method for evaluating the efficacy and toxicity of novel therapies for malignant glioma. Alcoholnonpreferring rats showed lower activity of glucose6phosphate dehydrogenase in the nucleus accumbens compared with. A number of rat species are used in research, contract research organizations and in the pharmaceutical industry including the wistar, sprague dawley and fischer 344. Seven of the rats constituted the sunexposed group and the other seven rats served as control. Ivermectin, a macrocyclic lactone, is commonly used against parasitic diseases in both animals and human beings. Comparisons of behavioral and neurochemical characteristics. The laboratory rat handbook of experimental animals. Main and interactive effects of fat and sodium contents of. The open field test oft was carried out on wistar and spraguedawley rats between the ages of 3 to 20 weeks. Although, its antiparasitic activity is well known, there is dearth of literature on its effect on the host. The rats were allowed to feed on commercially prepared r at.
Fourteen female wistar rats of average weight of 235 g used for the study were divided into 2 groups of 7 rats each. Neurochemical characteristics of the effects of delta. The effect of oleanolic acid on sperm motion characteristics and fertility of male wistar rats m. The rats were fasted overnight prior to blood collection.
Pdf on mar 1, 1998, b t clause published the wistar institute archives. Normal sodium normal fat nsnf, normal sodium high fat nshf, high sodium normal fat hsnf, high sodium high fat hshf diet for 12 weeks. He later established wistar institute in philadelphia that will have a major role in the development of the rat as an important laboratory animal similar to the impact the jackson laboratory had on the development of the laboratory mouse. Impact of temperature and the length of exposure on. At that time, the behavior of each naive rat was observed for two 3minute periods separated by an interval of 24 h day. Thirtyfive wistar rats were divided into three groups. Wistar institute in philadelphia, giving rise to the ubiquitous presentday wistar strain of rats festing, 1979. The characteristics of circadian rhythms acrophase, amplitude, and mesor of lipid peroxidation products thiobarbituric acid reactive substances and antioxidants reduced glutathione, glutathione peroxidase.
Doseresponse characteristics in spontaneously hypertensive and normotensive wistar rats. Yacon product pby modulates intestinal constipation and. At that time, the behavior of each naive rat was observed for two 3minute periods separated by an interval of 24 h. The present study on biochemical effects of ivermectin in wistar albino rats was undertaken to evaluate if it is toxic to the host. The laboratory rat, like its wild counterpart, is an extremely intelligent animal, probably more so than the other rodents commonly used for biomedical research. Macroscopic anatomy of the surface of the heart of albino wistar rats. Index of major rat strains, with histories and strain characteristics. Following primary and booster immunization, female wistar rats developed severe arthritis, with an incidence of 83% and low variability in clinical signs. Primate unit, south african medical research council, po box 19070, tygerberg 7505, south africa and2physiological sciences department, university of the western cape, pbag x17, bellville 7535, south africa. Twenty four adult male wistar rats divided into 4 groups of 6 animals eachwere.
The rat is the most commonly used rodent species for toxicology studies. Dawley from a hybrid hooded male and a female wistar rat. The results demonstrated that wistar rats were the most susceptible strain to cia followed by wistar furth rats, with sprague dawley rats being the least susceptible. Histochemical examination of the adrenal glands demonstrated that the 55 hyperplastic and 25 neoplastic lesions of the medulla usually had little or no chromafinity. Combination effect of laser diode for photodynamic therapy. Carbamazepine evoked reproductive toxicity in male wistar. Resistance between dexamethasonetreated and wistar fatty. The spermatozoa characteristics of wistar rats in groups a control, b treated with chloramphenicol for 20 days and c treated with chloramphenicol for 25 days are as presented in table i. Mortality and growth characteristics of rat strains commonly used in. Its longevity and high rate of spontaneous tumours make it an ideal. Pdf oxidative stress by tartrazine in the testis of wistar. Thirty male wistar rats procured from the central animal house, college of medicine, university of ibadan were used for this study. Limitations of the c6wistar rat intracerebral glioma model. Chlorophytum borivilianum safed musli root extract prevents.
Data are based on 50 carcinogenicity studies at uk sites of envigo for the period 19962018. Isoproterenolevoked renin release from the in situ perfused kidney. This is reflected in its behaviour and, when treated properly and sympathetically, its tolerance to handling and restraint. Evaluation of aphrodisiac activity of vigrx in male. Complete blood counts, differential white blood cell and platelet counts were performed on male and female bb wistar diabetic rats bbwd, their nondiabetic siblings bbwnd and outbred wistar rats of the line from which the bb wistar rats were derived. White paper september 2018 the wistar hannover rat for.
Comparative evaluation of the sperm characteristics and. Characteristics of spontaneously hypertensive, wistar. In the wistar rat strain, c6 cells implanted into the brain exhibit characteristics similar to spontaneous glioblastomas. Control c, constipated control cc, pby not constipated and constipated pby pbyc. The aim of this study was to evaluate the effects of a diet containing gm soybean meal rur on male and female wistar rats. Differences in the estrous cycles of gotokakizaki and wistar. Phenotypic characterization of type ii collageninduced. This process was repeated in other laboratories and today several strains of hypertensive rats with slightly different characteristics exist 3. Control group was orally administered with water alone while the experimental group was orally administered with tartrazine dissolved in water. The metabolic characteristics of the rats, including plas. This study was carried out to determine the growth performance of rats fed graded levels of corchorus olitorius supplemented diet.
Some functional characteristics of adrenal medullary. Methods animals and experimental design four weeks old gotokakizaki rats gkmoltac, aarhus colony and wistar han rats were purchased from taconic farms, inc. Characteristics of open field behavior of wistar and sprague. Pdf morphometrical characteristics of ageassociated. Pdf biochemical toxicity of ivermectin in wistar albino. It is the most widely used rat model for biomedical research. Mccormick, in a comprehensive guide to toxicology in nonclinical drug development second edition, 2017 wistar rats. Some functional characteristics of adrenal medullary tumors. Morphological characteristics and haematological studies. Growth curve reproduction data monogamous mating according robertson.
Hematological characteristics of the bb wistar rat wright. In search of an adequate model for the human metabolic syndrome, the metabolic characteristics of wistar rats were analysed after being submitted to different protocols of high fructose ingestion. Pharmaceutics free fulltext effect of food and an animal. Comparative characterization of rat strains wistar, wistarkyoto. The breeding of imported strains of isogenic rats was started in 1981 because of the lack of experimental rat models in brazil. Effect of vigrx on post ejaculatory interval in male albino wistar rats 29 8. Metabolic syndrome signs in wistar rats submitted to. The trial groups were classified into antemortem the exposure time of 20 min and postmortem groups exposure time until fatal outcome. It is characterized by its wide head, long ears, and a tail length that is always less than its body length.
The control rats were kept in cages at ambient temperature of 26c. These results suggest that thymus of aged wistar rats exhibits morphological characteristics similar to those found in aged human thymus and thus may serve as an. The development of todays laboratory rat has been essentially an american initiative and the great majority of the strains of these animals that are presently in use throughout the scientific world have originated in the usa. Genetic architecture of wistarkyoto rat and spontaneously. Biochemical and histopathological studies on female and male. Cytochemical characteristics of the brain of alcohol. Morphometrical characteristics of ageassociated changes. The breeding of imported strains of isogenic rats was started in 1981 because of the lack of experimental rat models in.
These findings indicate the low genetic variation in slc. The rats were divided into four groups a, b, c and d treated with graded doses 100, 200, 400 and 0 mgkg respectively of saponin extract of v. Characteristics of nonalcoholic fatty liver disease induced in wistar rats following four different diets nicole fakhourysayegh, 1 viviane traksmayra, 2 aline khazzaka, 3 fady esseily, 4, omar obeid, 5, may lahoudzouein, 6 and hassan younes 7 1 department of nutrition, faculty of pharmacy, saint joseph university, damascus road, beirut, lebanon 2 department of pathology, faculty of. The wistar rat is currently one of the most popular rats used for laboratory research. These data were compared to body weight and survival data previously collected for a sprague dawley strain crl. The spraguedawley strain came from crosses between wistar females and a hybrid male whose origins are unknown. The wistar bloodline has contributed more strains of rats than any other line. Whilst there are reported cases of laboratory and animal technicians contracting disease from laboratory rats these are relatively rare and most of them occurred several years ago before the intro duction of the highquality diseasefree animals which are widely available for use today.
Taconic sprague dawley rats are often used in studying metabolism and diabetes. Enzyme activities in the sensorimotor cortex did not change. We therefore investigated the effect of aqueous root extract of c. Lipasinbetatrophin is differentially expressed in liver and. Mar 22, 2016 understanding that a normal estrous cycle lasts 45 d in rats, at 6 months of age gotokakizaki rats had significantly more irregular cycles 90 19. Balbc mice have the characteristics of easy breeding and minimal weight variations. Limitations of the c6wistar rat intracerebral glioma. Together, these findings highlight the suitability of the wistar rat as an. Influence of garlic extract on temporal characteristics of. The c6 wistar model has been used extensively as a reproducible in vivo model for studying primary brain tumors including antiglioma immune responses. Its longevity and high rate of spontaneous tumours make it an ideal choice for ageing studies. The animals were exposed to 12 h light and 12 h dark. In order to provide a morphometrical description of the changes in the aged rat thymus and to relate them to apoptotic and proliferative activity of thymocytes, the thymuses from 3 and 18monthold male wistar rats and the percentages of bromodeoxyuridineincorporating and apoptotic cells in cultures of thymocytes were assessed by stereological analysis and flow cytometry, respectively.
Spermatozoa morphology and characteristics of male wistar rats. Cfhb wistar rats bred froman original stock supplied by carworth. These effects may be achieved through maintaining sperm antioxidant level which could be related to free. The rat is one of the most commonly used animal models in preclinical studies. Wistar rats 70 rats, 24 months of age and 45 rats, 30 months of age. Chromaffinity was found comparable to normal medulla in only three tumors examined histochemically. Chlorophytum borivilianum safed musli root extract. Journal of cell and animal biology sperm characteristics. Wistarst rats develop unilateral thyroid dysgenesis. High vitamin intakes during pregnancy and characteristics. Wistar rats and the high genetic similarity between the slc. The reproductive effects of saponin extract from vernonia amygdalina del.
Effect of vigrx on number of spermatogonia in male albino wistar rats 31 appendices i. Characterization of six rat strains rattus norvegicus by. The rats in group a control were administered with 0. Oct 24, 2019 male wistar rats n 48, initial body weight. Effect of carcinogen 1,2dimethylhydrazine treatment on. In order to standardize the terminology to describe mammary gland neoplasia, a sys temic classification of proliferative lesions has been developed based on an extensive retrospective survey of the light microscopic features of spontaneous mammary tumors from 2165 wistar rats. Characteristics of spontaneously hypertensive, wistar kyoto and munich wistar rats bred in brazil. Characteristics of open field behavior of wistar and spraguedawley rats yuzo asano safety research laboratory, tanabe seiyaku co. Reproductive characteristics of the female laboratory rat academic. Wild rats act as a reservoir for several zoonotic agents. Thirtysix adult male and female rats rattus norvegicus weighing.
Performance characteristics of male wistar rats fed graded. In male rats, the following characteristics were evaluated. Water and standard laboratory rat diet ashirwad pvt ltd, chandigarh, india were given ad libitum. The metabolism and toxicity of furosemide in the wistar rat and cd1 mouse. The objective of the present study is to provide in vivo evidence that the c6 rat glioma model is allogeneic. The sprague dawley rat and longevans rat were developed from wistar rats. Pdf spermatozoa morphology and characteristics of male. Chemical industry, osaka, japan, and male wistar rats weighing 200 to 280 g funabashi farm, chiba, japan were housed in individual cages in a temperaturecontrolled room with a 12hour lightdark cycle and free access to rodent food and water. Isoproterenolevoked renin release from the in situ perfused.
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